# Chapter 17 - The Genetic Code
- H. influenzae was the first genome sequenced (1995)
- E. coli was later sequenced (1997)
- Stop codons can be overridden
- ChIp uses a specific antibody to pull-down your protein of interest bound to specific DNA
- Genus → Species → Strain
- The redundancy found within the genetic code is actually well thought out
- Position 34 of the tRNA anticodon is often modified
- it is position 1 of the anti-codon
- may contain inosine
- Codons (61) → tRNA → Amino Acids (20-22)
- The important thing to note is that there are less tRNA than there are codons. There isn’t a unique tRNA for every single combination of bases ($4^3=64$). The ability for tRNA for cover multiple of these possible codons comes from the concept of the wobble position
**Supressor Mutations:**
- Intragenic: mutation at a different site in the same gene (within)
- Intergenic: mutation occurs in a different gene (across)
- Mutations can also occur in tRNA (uh oh, thats not good!)
- Neutral selection (example being SNPs)
- Open Reading Frame (ORF) is the region on the mRNA that encodes a polypeptide
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- The addition of an amino acid to a tRNA molecule requires an ATP
![[Chapter 17 - The Genetic Code.png|300]]
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