# Feb 25th Lecture Notes - DNA replication is unlikely to be coupled with eukaryotic transcription - RNA polymerase interacts with a single transcription factor in bacterial transcription but NOT eukaryotic transcription - Only prokaryotes have a region about 10 nucleotide upstream of +1 containing AT-rich base pairs - “DNA footprinting” is a technique that can be used to identify the specific binding site of a repressor, polymerase, or other protein on the DNA ([[DNA & protein interactions]]) - Specialized DNA repair systems are closlely linked to transcription. The biological rationale for this is likely because errors in the genetic information of actively transcribed genes if of greater immediate importance to the cell than errors in silent genes. Transcription-coupled DNA repair focuses repair on those DNA sequences that are being most heavily utilized by the cell. --- The correct order of transcriptional events after RNA polymerase binds to the promoter is: 1. Closed Complex Formation 2. Open Complex Formation 3. Start of RNA Synthesis 4. Promoter Clearance The three major steps in the transcription of a typical bacterial gene are: 1. Initiation (including abortive initation) which is the slowest step 2. Elongation, the fastest step but punctuated by pauses 3. Termination, which requires stalling and subsequent dissociation of the polymerase from DNA