# Split Sequencing ![[Split Sequencing Overview.png|400]] ## Split Pool Ligation-based Transcriptome sequencing (SPLiT-seq) https://neurobioinfo.github.io/_posts/splitseq https://www.genengnews.com/news/single-cell-rna-sequencing-may-be-split-by-parse-biosciences/ https://ucdavis-bioinformatics-training.github.io/2022-December-Single-Cell-RNA-Seq-Analysis/data_reduction/Expression_Matrix 1. Reverse Transcription - A special RT primer is designed with contains a well-specific barcode [?] What are the lengths of these fragments over the course of the experiment