> See also: > - [[Biotechnology]] # Chromatography **Chromatography** involves the separation of a substance by using a stationary phase (such as beads) and a mobile phase (sample and buffer). The analyte is the molecule being separated. The immobilized/stationary phase involves a type of bead which binds to the analyte and ## Affinity Chromatography In affinity chromatography, the column is filled with beads containing either ligands or receptors that are complementary to the protein being purified. The interact between the ## Ion-Exchange Chromatography ### Chromatography Machines ## Analyzing Data A **chromatogram**