> See also: > - [[DNA Manipulation]] # DNA Polymerases ### Polymerase Variations **Taq Polymerase** - Discovered from... - High heat resistance, making it useful for surviving the denaturing stage of polymerase chain reaction (PCR) ## Domains 3'->5' Exonuclease Proofreading Active Sites ## Reverse Transcriptase (RT) Reverse transcriptase (RT), also known as RNA-dependent RNA polymerase, is a DNA Since cDNA lacks introns, it can make mRNA that directly encodes a protein in bacteria without needing to be spliced, since bacteria are incapable of RNA splicing.