> See also: > - [[Genetics]] # Gene Mapping Gene mapping can help determine the location of genes/specific sequences within the larger scope of a given genome - Molecular weights of DNA sequences following restriction enzyme digestion - Probability of traits within a population when analyzing polygenic traits and recombination Homozygosity mapping Ex: 16-cM describes a gene with 16% recombination - centimorgan unit is defined as 1% - is it applicable to other species’ chromasomes (fruit flys)? linked [[Genes]] will more frequently undergo recombination together? **Microsatellite markers** - CsCl (salt) density gradient (origin of name, smaller peak on graph is “orbiting” larger one) - satellite DNA has more repetitive sequences, long stretches of DNA of a certain number of repeats of the same or highly similar sequences - These tend to be HIGHLY varied among individuals and also are not included in the coding regions - when we see two peaks (main and satellite marker) it implies potential heterozygosity - homozygotes don’t report recombination - Difficulty of [[Model Systems]] - Human gene mapping cannot rely on manifested traits, as it would require - Unlike fruit fly model organisms, humans cannot be experimentally inbred at high volumes to ensure the accuracy of the determined RF value -