# Nucleic Acid Sequencing ## Working With DNA vs RNA ### DNA Sequencing ### RNA Sequencing ## Barcodes & Unique Molecular Identifies (UMIs) | Read # | UMI | Mapping Position | Collapse? | | :----: | :------: | :--------------: | :-------: | | 1 | ATCGTACG | chr1:10123 | Yes | | 2 | ATCGTACG | chr1:10123 | Yes | | 3 | GCTAGCTA | chr1:10123 | No | | 4 | ATCGTACG | chr1:10123 | Yes | Despite having many reads following PCR/amplification, they only truly represent 2 molecules ## Sequencing Methods ![[Test Base.base]] --- | Technique | Description | | ----------------- | ----------- | | Sangar Sequencing | | | Nanopore | | ### Sangar Sequencing **Advantages** - Fast and cost effective (for low target number) **Disadvantages** ![[Figure-2_Coverage-graphs.svg|458]] ### Bulk vs Single Cell Sequencing ## Common Sequencing Errors ## Applications of Sequencing Techniques | Technique | Description | | ----------------------------- | ----------- | | [[ATAC Sequencing\|ATAC-Seq]] | | | | | | | |