# Programmed Cell Death --- **Pyroptosis** - Caspase-1 dependent cell death (inherently inflammatory) - Rare and in extreme circumstances **Necrosis** A far messier method of cell death that is ## Apoptosis **Apoptosis** The neat and ideal way for a cell to kill itself while also packaging up its remains in a way that is easy for [[Phagocytosis|phagocytic cells]] to clean up Apoptosis is a mechanism of cell death in which the cells to be killed are induced to degrade themselves from within, in a tidy manner. Sometimes referred to as "programmed cell death" ### Methods of Activation Programmed cell death protein 1 (PD-1) - Ligand on dying cell - Receptor on immune cell ## Cell Lysis If the proteins under investigation are inside of a cell, the first step of the process is destroy the cell membrane and other obstacles in the way Typically lysate should be kept on ice to prevent protein denaturation