> See also: > - Reference # Southern Blot "Dot Blot -> Southern Blot" is the DNA equivalent of "ELISA -> Western Blot" - **Reagents:** - Agarose Gel - Probes - Restriction Enzymes Probes are useful for locating SNPs and STRs which are both highly variable changes in genomes that can be associated with diseases: Both can be used for: - Pedigree/Inheritance Analysis - Disease Diagnosis - Genetic Markers when Mapping Genes - Paternity Testing **Restriction Fragment Length Polymorphisms (RFLP):** Involves analyzing *single nucleotide polymorphisms (SNPs)*, which change the restriction enzyme digestion site and thus pattern on a Southern blot. **Variable Number of Tandem Repeats (VNTR):** Involves analyzing *short tandem repeats (STRs)*, which are highly variable in the population. - The amount of repeats can *vary between the two alleles* within a single individual